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C-Peptide 1 (Rat) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-035-03 100 ul $275.00

ImageH-035-03-2451-3-H-500     H-035-03-2452-3-H-500
Protocol for Immunohistochemistry
 Tissue SampleHuman pancreas tissues
 Fixative10% Formalin 
 EmbeddingParaffin 
 Negative controlNo primary antibody 
 PretreatmentN/A
 Blocking

3% H2O2, 2% Normal Goat Serum

 Primary Antibody

Rabbit Anti-C Peptide-1 (Rat)  Antiserum (Catalog No.: H-035-03)

 Optimal Dilution

1:500, 1 hour at RT

 Secondary AntibodyGoat anti-Rabbit IgG, Biotinylated (1:400, 30 min) 
 Amplification

Streptavidin-HRP (Vector), 1:400, 30 min

 Detection systemHRP 
 SubstrateDAB (Sigma), 3 min 
 CounterstainedHematoxylin, 30 Sec
Catalog #H-035-03
FamilyInsulin
Standard Size100 ul
SequenceGlu - Val - Glu - Asp - Pro - Gln - Val - Pro - Gln - Leu - Glu - Leu - Gly - Gly - Gly - Pro - Glu - Ala - Gly - Asp - Leu - Gln - Thr - Leu - Ala - Leu - Glu - Val - Ala - Arg - Gln
SpeciesRat
HostRabbit
ImmunogenC Peptide, Insulin-1 (Rat)
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Alphabet_C_

035-03;035-53;H-035-53;

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