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Spexin / NPQ (Human, Mouse, Bovine) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-023-81 100 µl $450.00

Image

H-023-81-2487-3-H-500   H-023-81-2488-3-H-500

H-023-81-2487-3-R-500   H-023-81-2488-3-R-500

    

    

Protocol for Immunohistochemistry
 Tissue Sample

Human stomach tissues, stomach cancer tissues and rat stomach tissues

 Fixative10% Formalin 
 EmbeddingParaffin 
 Negative controlNo primary antibody 
 PretreatmentN/A
 Blocking2% Normal Goat Serum 
 Primary AntibodyRabbit Anti-Spexin (H) Antiserum (Catalog No.: H-023-81)
 Optimal Dilution

1:500, 1 hour at RT

 Secondary AntibodyGoat anti-Rabbit IgG, Biotinylated (1:400, 30 min) 
 Amplification

Streptavidin-HRP (Vector), 1:400, 30 min

 Detection systemHRP 
 SubstrateDAB (Sigma), 3 min 
 CounterstainedHematoxylin, 30 Sec
Catalog #H-023-81
FamilySpexin
Standard Size100 µl
SequenceAsn - Trp - Thr - Pro - Gln - Ala - Met - Leu - Tyr - Leu - Lys - Gly - Ala - Gln - NH2
SpeciesHuman, Mouse, Bovine
HostRabbit
ImmunogenSpexin / NPQ (Human, Mouse, Bovine)
ReconstitutionFor best and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Recommended Dilution FactorImmunohistochemistry - 1:500
Alphabet_S_
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