Spexin / NPQ (Human, Mouse, Bovine) - Antibody for Immunohistochemistry

Image

H-023-81-2487-3-H-500 H-023-81-2488-3-H-500

H-023-81-2487-3-R-500 H-023-81-2488-3-R-500

Protocol for Immunohistochemistry
Tissue Sample	Human stomach tissues, stomach cancer tissues and rat stomach tissues
Fixative	10% Formalin
Embedding	Paraffin
Negative control	No primary antibody
Pretreatment	N/A
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Spexin (H) Antiserum (Catalog No.: H-023-81)
Optimal Dilution	1:500, 1 hour at RT
Secondary Antibody	Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification	Streptavidin-HRP (Vector), 1:400, 30 min
Detection system	HRP
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-023-81

Family

Spexin

Standard Size

100 µl

Sequence

Asn - Trp - Thr - Pro - Gln - Ala - Met - Leu - Tyr - Leu - Lys - Gly - Ala - Gln - NH2

Species

Human, Mouse, Bovine

Host

Rabbit

Immunogen

Spexin / NPQ (Human, Mouse, Bovine)

Reconstitution

For best and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Recommended Dilution Factor

Immunohistochemistry - 1:500

Alphabet_S_

Please click here or click the reference tab on the top to see more articles that use this product.

Links to the publications that use this antibody:

Liu Y, Li S, Qi X, et al. A novel neuropeptide in suppressing luteinizing hormone release in goldfish, Carassius auratus.
Mol Cell Endocrinol. 2013. doi:10.1016/j.mce.2013.04.008

Porzionato A, Rucinski M, Macchi V, et al. Spexin is expressed in the carotid body and is upregulated by postnatal hyperoxia exposure.
Adv Exp Med Biol. 2012;758:207-13.