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NPFF-1R (185-203) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-001-58 100 µl $299.00

Image


Western Blot Analysis 

 Tissue Sample Rat brain tissue homogenate
 Electrophoresis 10% SDS-PAGE gel
 Negative control No primary antibody
 Blocking 1% BSA in TBST or 5% dry milk in TBST
 Primary Antibody Rabbit Anti-NPFF-1R (185-203) (Human) Antiserum (Catalog No.:H-001-58)
 Optimal Dilution 1:200 ( overnight at 4oC)
 Secondary Antibody Donkey anti-Rabbit IgG whole (H+L), HRP conjugate(1:5000, 30 min)
 Detection system HRP
 Substrate SuperSignal Chemiluminescent Substrate
    

Protocol for Immunohistochemistry:

 Tissue Sample

 Rat & Mouse Brain

 Fixative

 10% formalin

 Embedding

 Paraffin

 Negative Control

 No primary antibody

 Pretreatment

 Intact

 Blocking

 2% Normal Goat Serum

 Primary Antibody

 Rabbit Anti-NPFF1R (185-203) (H) Serum (Catalog No.:H-001-58)

 Optimal Dilution

 1:200 (1 hour at RT)

 Secondary Antibody

 Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)

 Amplification

 ABC (Vector) (1:400, 30 min)

 Detection System

 HRP

 Substrate

 DAB (Sigma), 3 min

 Counterstained

 Hematoxylin, 30 Sec

Catalog #H-001-58
Standard Size100 µl
SequenceGlu - Glu - His - His - Phe - Met - Val - Asp - Ala - Arg - Asn - Arg - Ser - Tyr - Pro - Leu - Tyr - Ser - Cys
SpeciesHuman
HostRabbit
ReconstitutionFor consistent and reproducible results, reconstitute with 50ul of distilled water for the equivalent of undiluted antiserum, immediately before use. Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 100ul of rabbit anti- NPFF-1R (185-203) (H) serum in the lyophilized form.
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