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Urotensin II, Prepro (21-40) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-071-24 100 µl $450.00

Image

Protocol for Immunohistochemistry
 Tissue Sample Human placenta
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control Pre-immuno serum
 Pretreatment Intact
 Blocking 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-Urotensin II, Prepro (21-40) (Human) Serum (Catalog No.:H-071-24)
 Optimal Dilution 1:500 (over night at 4°C)
 Secondary Antibody  Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification  ABC (Vector) (1:400, 30 min)
 Detection system HRP
 Substrate DAB (Sigma), 3 min
 Counterstained Hematoxylin, 30 Sec
Catalog #H-071-24
Standard Size100 µl
SequenceLeu - Pro - Leu - Leu - Asp - Ser - Arg - Glu - Ile - Ser - Phe - Gln - Leu - Ser - Ala - Pro - His - Glu - Asp - Ala
SpeciesHuman
ReconstitutionReconstitute with 100 ul distilled water.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Recommended Dilution Factor1: 500 (immunohistochemistry)
1: 3200 (indirect ELISA)
Cross ReactivityThis antibody is crossreactivity with human Urotensin II, Prepro (21-40). It was tested in human placenta tissues in immunohistochemistry
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