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Musclin / Osteocrin (Ostn) (31-77) (Rat) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-028-72 50 µl $450.00

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Protocol for Immunohistochemistry: 
 Tissue Sample Rat/Mouse heart tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment Target retrieval 25 min (Steam)
 Blocking 2% Normal Goat Serum
 Primary Antibody Anti-Musclin (31-77) (Rat) Serum (Catalog No.:H-028-72)
 Optimal Dilution 1:200 (1hour at RT)
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification  ABC (Vector)(1:400, 30 min)
 Detection system HRP
 Substrate DAB (Sigma), 3 min
 Counterstained Hematoxylin, 30 Sec
Catalog #H-028-72
Standard Size50 µl
SpeciesRat
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
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