Vasohibin [Cys0] (286-299) (Human) - Antibody for Immunohistochemistry

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Protocol for Immunohistochemistry:
Tissue Sample	Human placenta tissue
Fixative	10% formalin
Embedding	Paraffin
Negative Control	No primary antibody
Pretreatment	Intact
Blocking	2% Normal Goat Serum
Primary Antibody	Rabbit Anti-Vasohibin (286-299) (Human) Antiserum (Catalog No.:H-002-96)
Optimal Dilution	1:500 (overnight at 4 ^oC)
Secondary Antibody	Goat anti-Rabbit IgG, HRP conjugated
Substrate	DAB (Sigma), 3 min
Counterstained	Hematoxylin, 30 Sec

Catalog #

H-002-96

Standard Size

100 µl

Sequence

Cys - Gly - Arg - Asp - Asp - Phe - Arg - Lys - Glu - Leu - Glu - Arg - His - Ala - Arg

Species

Human

Storage Condition

Please store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.

Content

100 ul NET Rabbit Antiserum in Lyophilized powder without NaN3.

Cross Reactivity

This antibody is crossreactivity with human Vasohibin (286-299) at 100%. It was tested in immunohistochemistry in human placenta tissues. This antibody does not cross react with Human Vasohibib (336-365).

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