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GPR-39 (423-453) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-002-85 100 µl $375.00

Image

Western Blot Analysis

 Sample Mouse and rat brain tissue homogenate
 Electrophoresis 10% SDS-PAGE gel, reduce condition
 Negative control 100oC for 5 min
 Denatured sample  1% BSA in TBST
 Primary Antibody Rabbit Anti-GPR 39 (423-453) (Human) Antibody (Catalog No.:H-002-85)
 Optimal Dilution 1: 500 (overnight at 4 oC)
 Secondary Antibody Rabbit anti-rabbit IgG, HRP conjugated
 Substrate ECL
 Positive Band  ~ 50 KDa

Protocol for Immunohistochemistry:

 Tissue Sample

 Rat & Mouse brain tissue

 Fixative

 10% formalin

 Embedding

 Paraffin

 Negative Control

 No primary antibody

 Pretreatment

 Intact

 Blocking

 2% Normal Goat Serum

 Primary Antibody

 Rabbit Anti-GPR 39 (423-453) (Human) Antiserum (Catalog No.:H-002-85)

 Optimal Dilution

 1:500 (overnight at 4 oC)

 Secondary Antibody

 Goat anti-Rabbit IgG, HRP conjugated

 Substrate

 DAB (Sigma), 3 min

 Counterstained

 Hematoxylin, 30 Sec

Catalog #H-002-85
Standard Size100 µl
SpeciesHuman
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
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