中文网站正在持续更新中,请密切关注我们康肽生物的最新动态,或点击访问右上角的英文官方网站 www.phoenixpeptide.com
PHOENIX PHARMACEUTICALS, INC. TOP HOME PAGE
Top » Catalog » Antibodies » For Immunohistochemistry » H-013-01 English Version | My Account | 联系我们 | China



 多肽



 标记多肽 



 多肽激素文库



 抗体 
   免疫组化抗体
   纯化免疫球蛋白 
   抗体标记 



 免疫试剂盒 



 生物标志物阵列 



 多肽样品检测



 自定义肽链合成及GMP



 产品目录索取



 样品准备



 提问和解答


DNP (Green Mamba) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-013-01 50 µl $350.00

Image

Immunostaining for Dendroaspis natriuretic peptide. Left, Normal human heart. Middle, Human with congestive heart failure (CIIF). Right, Staining with nonimmune response serum (NRS) from same heart as shoen in middle panel.Schirger et al. Mayo Clin Proc. 1999 Feb;74(2):126-30.

Protocol for Immunohistochemistry

Immunohistochemical studies were performed by the indirect immunoperoxidase method. Tissues were immediately fixed with 10% buffered formalin and embedded in paraffin; sections 6 μm thick were cut and mounted on silanized glass slides. The slides were incubated at 60°C and deparaffinized with graded concentrations of xylene and ethanol. To block the activity of endogenous peroxidase, we incubated the slides with 0.6% hydrogen peroxide in methanol for 20 minutes at room temperature. After being washed, sections were incubated in 5% goat serum (Dako Corp., Carpinteria, California) for 10 minutes at room temperature to reduce non-specific background staining, and they were then incubated with polyclonal rabbit anti-DNP (Phoenix Pharmaceuticals) antiserum at a dilution of 1:500 (in normal goat serum) in humidified chambers for 24 hours at room temperature. All slides were incubated for 30 minutes with a second antibody-horseradish peroxidase conjugate (BioSource, Camarillo, California). The reaction was visualized by incubating the sections with freshly prepared reagent that contained 3′-amino-9′-ethylcarbazole (Sigma Chemical Company, St. Louis, Missouri) in dimethylformamide and sodium acetate. The sections were counter-stained with hematoxylin, coverslipped, and reviewed with use of an Olympus microscope. Six independent observers, without knowledge of the respective groups from which these tissues originated, reviewed these sections. The presence of DNP-LI was quantified on the basis of the following scale of staining: 0=none; 1=minimal density; 2=mild density; 3=moderate density; and 4=maximal density. Control sections were stained with 1% non-immune goat serum

Schirger et al. Mayo Clin Proc. 1999 Feb;74(2):126-30.

Catalog #H-013-01
Standard Size50 µl
SequenceGlu - Val - Lys - Tyr - Asp - Pro - Cys - Phe - Gly - His - Lys - Ile - Asp - Arg - Ile - Asn - His - Val - Ser - Asn - Leu - Gly - Cys - Pro - Ser - Leu - Arg - Asp - Pro - Arg - Pro - Asn - Ala - Pro - Ser - Thr - Ser - Ala

Disulfide Bridge: Cys7 - Cys23
Specific SpecieGreen Mamba
HostRabbit
ReconstitutionFor best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. For the antiserum, add additional buffer. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis contains 50µl of Rabbit Anti-DNP Serum in the lyophilized form.
Recommended Dilution FactorImmunofluorescence: 1:200
PAP or ABC 1:500
Cross Reactivity
Peptide % Cross-reactivity 
Dendroaspis Natriuretic Peptide (DNP) 100
α-ANP (1-28) (Human, Canine) 
Prepro-ANF (26-55) (Human) 
CNP-22 (Human, Porcine, Rat) 
BNP-32 (Human)  
Alphabet_D_
Please click here or click the reference tab on the top to see more articles that use this product.
分类搜索
关键字搜索
按字母搜索
A B C D E F G H I J K L M N
O P Q R S T U V W X Y Z

Copyright © 2024 PHOENIX BIOTECH