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Amylin (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-017-03 50 l $175.00

Image

Protocol for Immunohistochemistry 
 Tissue SampleHuman Pancreas tissue
 Fixative10% Formalin 
 EmbeddingParaffin
 Negative controlNo primary antibody 
 PretreatmentTarget retrieval 10 min (Steam)*
 Blocking2% Normal Goat Serum 
 Primary AntibodyRabbit Anti-Amylin (Human) Purified IgG (Catalog No:H-017-03)
 Optimal Dilution 1:200 (1hour at RT)
 Secondary AntibodyGoat anti-Rabbit IgG, Biotinylated (1:400, 30 min) 
 Amplification ABC (Vector) (1:400, 30 min)
 Detection systemHRP 
 SubstrateDAB (Sigma), 3 min 
 CounterstainedHematoxylin, 30 Sec 
* Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Catalog #H-017-03
Standard Size50 l
SequenceLys - Cys - Asn - Thr - Ala - Thr - Cys - Ala - Thr - Gln - Arg - Leu - Ala - Asn - Phe - Leu - Val - His - Ser - Ser - Asn - Asn - Phe - Gly - Ala - Ile - Leu - Ser - Ser - Thr - Asn - Val - Gly - Ser - Asn - Thr - Tyr - NH2
SpeciesHuman
HostRabbit
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 50µl of Rabbit Anti-Amylin (Human) Antibody Serum in the lyophilized form.
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