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Humanin, Nuclear-encoded / HN(N) (Rat) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-018-28 50 µl $450.00

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Protocol for Immunohistochemistry
Tissue Sample Rat heart
Fixative 10% Formalin 
Embedding Paraffin 
Negative control No primary antibody 
Pretreatment Target Retrieval 10 min (micro-wave oven)
Primary Antibody Humanin (N) (Rat) Antibody
Optimal Dilution 1:500 (2-3hour at RT)
Secondary Antibody Goat Anti-Rabbit IgG -HRP  
Substrate DAB
Counterstained Hematoxylin

Catalog #H-018-28
Standard Size50 µl
SequenceMet - Ala - Thr - Arg - Gly - Phe - Asn - Cys - Leu - Leu - Leu - Ser - Ile - Ser - Glu - Ile - Asp - Leu - Pro - Val - Lys - Arg - Leu - Glu - Ser - Pro - Asn - Lys - Thr - Arg - Arg - Pro - Tyr - Gly - Ala - Ser - Ile - Tyr
SpeciesRat
HostRabbit
ReconstitutionFor best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 50µl of Rabbit Anti-Humanin (N) (Rat) Serum in the lyophilized form.
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