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Corticotropin Releasing Factor (CRF) (Human, Rat, Mouse, Canine, Feline) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-019-06 50 µl $175.00

Image

Protocol for Immunohistochemistry: 
Tissue Sample Rat hypothalamus
Fixative 10% Formalin 
Embedding Paraffin 
Negative control No primary antibody 
Pretreatment Target Retrieval 25 min (Steam)
Blocking 2% Normal Goat Serum
Primary Antibody Anti-Corticotropin Releasing Factor (CRF) (Human, Rat) Serum
Optimal Dilution 1:100
Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400)
Amplification  ABC (Vector) 
Detection system HRP 
Substrate DAB (Sigma)
Counterstained Hematoxylin 

Catalog #H-019-06
Standard Size50 µl
SequenceSer - Glu - Glu - Pro - Pro - Ile - Ser - Leu - Asp - Leu - Thr - Phe - His - Leu - Leu - Arg - Glu - Val - Leu - Glu - Met - Ala - Arg - Ala - Glu - Gln - Leu - Ala - Gln - Gln - Ala - His - Ser - Asn - Arg - Lys - Leu - Met - Glu - Ile - Ile - NH2
SpeciesHuman, Rat, Mouse
HostRabbit
ReconstitutionFor best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 50µl of Rabbit Anti-Corticotropin Releasing Factor (Human, Rat) Serum in the lyophilized form.
Cross Reactivity
Peptide %Cross-reactivity 
CRF (Human, Rat) 100
ACTH (Human) 
LH-RH  
PACAP-38 (Human, Rat, Ovine)  
[Arg8]-Vasopressin 
Urocortin (Human)  
Urocortin (Rat) 
BNP-45 (Rat) 
Alphabet_C_
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