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Coupling Factor 6 (CF6) Amide (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-007-53 50 µl $450.00

Image

 

Protocol for Immunohistochemistry
 Tissue Sample Rat Heart
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 PretreatmentPronase
 Blocking2% Normal Goat Serum
 Primary AntibodyAnti-Coupling Factor 6 (Human) serum (Catalog No.:H-007-53)
 Optimal Dilution1: 100
 Secondary Antibody Goat Anti-Rabbit IgG, Biotinylated (1:400) 
 AmplificationABC (Vector)
 Detection systemHRP 
 SubstrateDAB (Sigma), 3 min
 CounterstainedHematoxylin

Catalog #H-007-53
Standard Size50 µl
SequenceAsn - Lys - Glu - Leu - Asp - Pro - Ile - Gln - Lys - Leu - Phe - Val - Asp - Lys - Ile - Arg - Glu - Tyr - Lys - Ser - Lys - Arg - Gln - Thr - Ser - Gly - Gly - Pro - Val - Asp - Ala - Ser - Ser - Glu - Tyr - Gln - Gln - Glu - Leu - Glu - Arg - Glu - Leu - Phe - Lys - Leu - Lys - Gln - Met - Phe - Gly - Asn - Ala - Asp - Met - Asn - Thr - Phe - Pro - Thr - Phe - Lys - Phe - Glu - Asp - Pro - Lys - Phe - Glu - Val - Ile - Glu - Lys - Pro - Gln - Ala - NH2
SpeciesHuman
HostRabbit
ReconstitutionFor consistent and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 50µl of Rabbit Anti- Coupling Factor 6 [CF 6
Precursor (33-108)-NH2] Human) Serum in the lyophilized form.
Recommended Dilution FactorABC: 1:100
Western blot: 1: 500
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