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Urocortin (Rat, Mouse) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-019-15 50 µl $300.00

Image

Protocol for Immunohistochemistry
 Tissue SampleRat Piriform Cortex
 Fixative10% Formalin 
 EmbeddingParaffin 
 Negative controlNo primary antibody 
 PretreatmentN/A
 Blocking

3% H2O2, 2% Normal Goat Serum

 Primary Antibody

Rabbit Anti-Urocortin (Rat) (Human) Antibody (Catalog No.: H-019-15)

 Optimal Dilution

1:100

 Secondary AntibodyGoat anti-Rabbit IgG, Biotinylated (1:400, 30 min) 
 Amplification

Streptavidin-HRP (Vector), 1:400, 30 min

 Detection systemHRP 
 SubstrateDAB (Sigma), 3 min 
 CounterstainedHematoxylin, 30 Sec
Catalog #H-019-15
Standard Size50 µl
SequenceAsp - Asp - Pro - Pro - Leu - Ser - Ile - Asp - Leu - Thr - Phe - His - Leu - Leu - Arg - Thr - Leu - Leu - Glu - Leu - Ala - Arg - Thr - Gln - Ser - Gln - Arg - Glu - Arg - Ala - Glu - Gln - Asn - Arg - Ile - Ile - Phe - Asp - Ser - Val - NH2
SpeciesRat, Mouse
HostRabbit
ImmunogenUrocortin (Rat)
ReconstitutionFor best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 50µl of Rabbit Anti-Urocortin (Rat) Serum in the lyophilized form.
Recommended Dilution FactorImmunofluorescence: 1:200
PAP or ABC: 1:500
Cross Reactivity
Peptide % Cross-reactivity 
Urocortin (Rat, Mouse) 100
Urocortin (Human)100
Cst-14 (Rat) 1.94
CRF (Human, Rat) 0.0015
MCH (Human, Mouse, Rat)  0
LH-RH (Human, Porcine, Rat) 
Somatostatin  
NPY (Human, Rat) 
Alphabet_U_
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