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Urocortin II (Mouse) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-019-24 50 µl $450.00

Image

Protocol for Immunohistochemistry:
Tissue Sample Mouse Brain
Fixative 10% Formalin 
Embedding Paraffin 
Negative control No primary antibody 
Pretreatment Target Retrieval 25 min (Steam)  
Blocking 2% Normal Goat Serum 
Primary Antibody Anti-Urocortin II (Mouse) (Catalog No: H-019-24)
Optimal Dilution 1: 50~100
Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400) 
Amplification  ABC (Vector) 
Detection system  HRP
Substrate DAB (Sigma)
Counterstained Hematoxylin

Catalog #H-019-24
Standard Size50 µl
SequenceVal - Ile - Leu - Ser - Leu - AspVal - Pro - Ile - Gly - Leu - Leu - Arg - Ile - Leu - Leu - Glu - Gln - Ala - Arg - Tyr - Lys - Ala - Ala - Arg - Asn - Gln - Ala - Ala - Thr - Asn - Ala - Gln - Ile - Leu - Ala - His - Val - NH2
SpeciesMouse
HostRabbit
ImmunogenUrocortin II (Mouse)
ReconstitutionFor best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 50µl of Rabbit Anti-Urocortin II (Mouse) Serum in the lyophilized form.
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