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Urocortin II (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-019-30 50 µl $450.00

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Protocol for Immunohistochemistry:
Tissue Sample Human pancreas tissue
Fixative 10% Formalin 
Embedding Paraffin 
Negative control No primary antibody 
Pretreatment Intact
Blocking 2% Normal Goat Serum 
Primary Antibody Rabbit Anti-Urocortin II (Human) Antiserum (Catalog No.:H-019-30)
Optimal Dilution 1:500 (overnight at 4oC)
Secondary Antibody Goat anti-Rabbit IgG, HRP conjugated
Substrate DAB (Sigma), 10"
Counterstained Hematoxylin, 30 Sec

Catalog #H-019-30
Standard Size50 µl
SequenceIle - Val - Leu - Ser - Leu - Asp - Val - Pro - Ile - Gly - Leu - Leu - Gln - Ile - Leu - Leu - Glu - Gln - Ala - Arg - Ala - Arg - Ala - Ala - Arg - Glu - Gln - Ala - Thr - Thr - Asn - Ala - Arg - Ile - Leu - Ala - Arg - Val - NH2
SpeciesHuman
HostRabbit
ImmunogenUrocortin II (Human)/ SRP (6-43)
ReconstitutionFor best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 50µl of Rabbit Anti- Urocortin II (Human) Serum in the lyophilized form. The HPLC purified standard peptide, Urocortin II (Human) can be detected with this antiserum at 1: 12800 (0.078μl/ml) in ELISA.
Recommended Dilution FactorDirect ELISA: 1: 3200
Immunohistochemistry: 1:500
Alphabet_U_
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