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ANP, alpha (1-28) (Human, Porcine, Ovine, Canine) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-005-06 50 µl $175.00

Image   
Protocol for Immunohistochemistry:
 Tissue Sample Rat or Mouse heart tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment Intact
 Blocking 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-ANP-alpha (1-28) (Human) (Catalog No.:H-005-06)
 Optimal Dilution 1:500
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification
 ABC (Vector) (1:400, 30 min)
 Detection system HRP
 Substrate DAB (sigma), 30min
 Counterstained Hematoxylin, 30Sec
Catalog #H-005-06
FamilyANP
Standard Size50 µl
SequenceSer - Leu - Arg - Arg - Ser - Ser - Cys - Phe - Gly - Gly - Arg - Met - Asp - Arg - Ile - Gly - Ala - Gln - Ser - Gly - Leu - Gly - Cys - Asn - Ser - Phe - Arg - Tyr

Disulfide Bridge: Cys7 - Cys23
SpeciesHuman, Canine, Ovine, Porcine
HostRabbit
ReconstitutionFor consistent and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 50µl of rabbit anti- Atrial Natriuretic Polypeptide
(a-ANP) (1-28) (Human, Canine, Porcine, Bovine) Serum in the lyophilized form.
Recommended Dilution FactorImmunofluorescence- 1:200
ABC- 1:500
Cross Reactivity
Peptide % Cross-reactivity 
 a-ANP (1-28) (Human, Ovine, Canine) 100
 b-ANP (Human 100
 Urodilatin 100
 ANP (Rat) 0.23
 BNP-32 (Human)  0.024
 Endothelin-1 (Human, Rat, Porcine, Mouse, Canine, Bovine) 0.01
 CNP (24-53)  0.009
 BNP-26 (Porcine) 0.006
 BNP-45 (Rat) 0
Alphabet_A_
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