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Endothelin-1 (ET-1) (145-162)-Amide, Prepro (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-023-05 100 µl $450.00

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Protocol for Immunohistochemistry
 Tissue Samplemouse & rat kidney tissue
 Fixative10% Formalin 
 EmbeddingParaffin
 Negative controlPre-immuno serum
 PretreatmentIntact 
 Blocking2% Normal Goat Serum 
 Primary AntibodyRabbit Anti-Endothelin-1, prepro (145-162)(Human) Serum (Catalog No.:H-023-05) 
 Optimal Dilution1:500 (over night at 40C)
 Secondary AntibodyGoat anti-Rabbit IgG, Biotinylated (1:400, 30 min) 
 Amplification ABC (Vector) (1:400, 30 min)
 Detection systemHRP 
 SubstrateDAB (Sigma), 3 min 
 CounterstainedHematoxylin, 30 Sec

Catalog #H-023-05
Standard Size100 µl
SequenceGly - Lys - Asp - Cys - Ser - Lys - Leu - Gly - Lys - Lys - Cys - Ile - Tyr - Gln - Gln - Leu - Val - Arg - NH2
SpeciesHuman
HostRabbit
ReconstitutionFor consistent and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum immediately before use. For the antiserum, add additional buffer.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 100µl of rabbit anti-Endothelin-1 (145-162) (Human) serum in the lyophilized form.
Recommended Dilution FactorDirect ELISA: 1:3200
Immonohistochemistry (ABC) 1:500
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