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Leptin (Rat) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-003-17 100 µl $350.00

Image

Western Blot Analysis of Vaspin in rat visceral white adipose tissue 

 Tissue Sample Rat visceral white adipose tissue homogenate  
 Electrophoresis 15% Tricine-PAGE gel
 Negative control No primary antibody
 Blocking 1% BSA in TBST or 5% dry milk in TBST
 Primary Antibody Rabbit Anti-Leptin (Rat) Antiserum (Catalog No.:H-003-17)
 Optimal Dilution 1:500-~1000 (4 hour at RT, or overnight at 4° C)
 Secondary Antibody Donkey anti-Rabbit IgG whole (H+L), HRP conjugate(1:5000, 30 min)
 Detection system HRP
 Substrate SuperSignal Chemiluminescent Substrate

     

Protocol for Immunohistochemistry

  Tissue Sample Mouse and Rat visceral white adipose tissue homogenate

 Fixative

 10% Formalin

 Embedding Paraffin

 Negative control

 No primary antibody

 Pretreatment

 Intact

 Blocking

 2% Normal Goat Serum

 Primary Antibody

 Rabbit Anti-Leptin ( Rat) Serum (Catalog No.:H-003-17)

 Optimal Dilution

 1:500~1000 (1 hour at RT)

 Secondary Antibody

 Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)

 Amplification

 ABC (Vector) (1:400, 30 min)

 Detection system

 HRP

 Substrate

 DAB (Sigma), 3 min

 Counterstained

 Hematoxylin, 30 Sec

Catalog #H-003-17
Standard Size100 µl
SpeciesRat
HostRabbit
ReconstitutionFor consistent and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThe contents of this vial have been accurately determined to contain 100µl of rabbit anti-Leptin (Rat) (Recombinant) serum in the lyophilized form
Recommended Dilution Factor1: 500 ~ 1000 (western blot)
1: 12800 (indirect ELISA)
1: 500~1000 (Immunohistochemistry-ABC)
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