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Leptin (Mouse) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-003-13 100 µl $350.00

Image

Protocol for Immunohistochemistry  

Tissue Sample

 Mouse and Rat visceral white adipose tissue homogenate

Fixative

 10% Formalin

Embedding Paraffin

Negative control

 No primary antibody

Pretreatment

 Intact

Blocking

 2% Normal Goat Serum

Primary Antibody

Rabbit Anti-Leptin ( Mouse) Serum (Catalog No.:H-003-13)

Optimal Dilution

 1:500~1000 (1 hour at RT)

Secondary Antibody

 Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)

Amplification

 ABC (Vector) (1:400, 30 min)

Detection system

 HRP

Substrate

 DAB (Sigma), 3 min

Counterstained

 Hematoxylin, 30 Sec 


Catalog #H-003-13
Standard Size100 µl
SpeciesMouse
HostRabbit
ReconstitutionFor consistent and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum, immediately before use
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 100µl of rabbit anti-Leptin (Mouse) serum in the lyophilized form.
Recommended Dilution Factor1:500-1000 (ABC Immunohistochemistry)
1: 12800 (indirect ELISA)
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