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GLP-1 (7-36)-Amide (Human, Rat, Mouse, Porcine, Bovine, Canine, Ovine) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-028-11 50 µl $325.00

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Protocol for Immunohistochemistry
 Tissue Sample

Human pancreas tissues

 Fixative10% Formalin 
 EmbeddingParaffin 
 Negative controlNo primary antibody 
 PretreatmentN/A
 Blocking

3% H2O2, 2% Normal Goat Serum

 Primary Antibody

Rabbit Anti-GLP-1 (7-36)-NH2 Antiserum (Catalog No.: H-028-11)

 Optimal Dilution

1:500, 1 hour at RT

 Secondary AntibodyGoat anti-Rabbit IgG, Biotinylated (1:400, 30 min) 
 Amplification

Streptavidin-HRP (Vector), 1:400, 30 min

 Detection systemHRP 
 SubstrateDAB (Sigma), 3 min 
 CounterstainedHematoxylin, 30 Sec
Catalog #H-028-11
FamilyGLP-1
Standard Size50 µl
SequenceHis - Ala - Glu - Gly - Thr - Phe - Thr - Ser - Asp - Val - Ser - Ser - Tyr - Leu - Glu - Gly - Gln - Ala - Ala - Lys - Glu - Phe - Ile - Ala - Trp - Leu - Val - Lys - Gly - Arg - NH2
SpeciesHuman, Rat, Porcine, Mouse, Bovine, Ovine, Canine
HostRabbit
ImmunogenGlucagon-Like-Peptide-1 (7-36)-Amide
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentEach vial contains 50µl of rabbit Anti-Glucagon-Like Peptide-1 (7-36) Amide (GLP-1) [Prepro-Glucagon (78-107) Amide] (Human) serum in the lyophilized form. Reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum. For diluted antiserum add additional buffer.
Cross Reactivity
Peptide % Crossreactivity 
GLP-1 (7-36) (Human, Rat, Mouse) 100
GLP-1 (7-37) (Human, Rat, Mouse) 0.4 
GLP-2 (Human) 
Glucagon (Human)
Insulin (Human) 
Secretin (Porcine)
GIP (Human)  
VIP (Human, Porcine, Rat) 
Alphabet_G_
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