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PK2L (48-68) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-002-78 100 µl $450.00

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 Protocol for Immunohistochemistry
  Tissue Sample  Rat and mouse brain tissue
 Fixative 10% Formalin
 Negative control No primary antibody
 Pretreatment Target retrieval 25 min (Steam)
 Blocking 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-PK 2 L (48-68) (Human) Serum (Catalog No.:H-002-78)
 Optimal Dilution 1:200 (overnight at cold room)
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification  ABC (Vector) (1:400, 30 min)
 Detection system HRP
 Substrate DAB (Sigma), 3 min
 Counterstained Hematoxylin, 30 Sec 

Catalog #H-002-78
Standard Size100 µl
SequenceAsn - Asn - Phe - Gly - Asn - Gly - Arg - Gln - Glu - Arg - Arg - Lys - Arg - Lys - Arg - Ser - Lys - Arg - Lys - Lys - Glu
SpeciesHuman
HostRabbit
ReconstitutionFor consistent and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum immediately before use. Reconstituted antibody can also be aliquotted (5~10ul) and stored frozen at
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