中文网站正在持续更新中,请密切关注我们康肽生物的最新动态,或点击访问右上角的英文官方网站 www.phoenixpeptide.com
PHOENIX PHARMACEUTICALS, INC. TOP HOME PAGE
Top » Catalog » Antibodies » For Immunohistochemistry » H-028-13 English Version | My Account | 联系我们 | China



 多肽



 标记多肽 



 多肽激素文库



 抗体 
   免疫组化抗体
   纯化免疫球蛋白 
   抗体标记 



 免疫试剂盒 



 生物标志物阵列 



 多肽样品检测



 自定义肽链合成及GMP



 产品目录索取



 样品准备



 提问和解答


GLP-1 (7-37) (Human, Rat, Mouse, Porcine, Bovine, Canine, Ovine) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-028-13 50 µl $150.00

Image

Rat pancreatic and intestinal sections with an antiserum for human GLP-17–37 (Phoenix Pharmaceuticals, Mountain View, California, USA) at a concentration of 1/500. The second antibody was a biotinylated goat anti-rabbit immunoglobulin, and the signal was detected using the Vectastain ABC kit (Vector Laboratories, Burlingame, California, USA) with DAB as a substrate. The section was counterstained with methyl green. Nie et al. J Clin Invest. 2000 Apr;105(7):955-65.

Protocol for Immunohistochemistry: 
 Tissue Sample Rat Pancreatic and intestinal tissue  
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control Pre-immuno serum
 Pretreatment Intact
 Blocking 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-GLP-1 (7-37) (Human, Rat, Mouse, Porcine) Serum
(Catalog No.:H-028-13)
 Optimal Dilution 1:500 (1hour at RT)
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification  ABC (Vector) (1:400, 30 min)
 Detection system HRP
 Substrate DAB (Sigma), 3 min
 Counterstained

 with methyl green

Catalog #H-028-13
Standard Size50 µl
SequenceHis - Ala - Glu - Gly - Thr - Phe - Thr - Ser - Asp - Val - Ser - Ser - Tyr - Leu - Glu - Gly - Gln - Ala - Ala - Lys - Glu - Phe - Ile - Ala - Trp - Leu - Val - Lys - Gly - Arg - Gly
SpeciesHuman, Rat, Mouse, Porcine, Bovine, Ovine, Canine
HostRabbit
ReconstitutionFor best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 50µl of Rabbit Anti-Glucagonl-Like Peptide-1 (7-37) (Human, Rat, Mouse) Serum in the lyophilized form.
Recommended Dilution FactorIndirect ELISA: 1: 3200
Immunohistochemistry: 1~500
Cross Reactivity
Peptide % Crossreactivity 
 GLP-1 (7-37) (Human) 100
 GLP-1 (Human)  100
 GLP-1 (7-36) (Human) 0
 GLP-2 (Human) 0
 Glucagon (Human) 0
 Insulin (Human) 0
 Secretin (Porcine) 0
 GIP (Human) 0
 VIP (Human, Porcine, Rat) 0
 GH-RF (Human) 0
Alphabet_G_
Please click here or click the reference tab on the top to see more articles that use this product.
分类搜索
关键字搜索
按字母搜索
A B C D E F G H I J K L M N
O P Q R S T U V W X Y Z

Copyright © 2024 PHOENIX BIOTECH