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FGF-21 [Cys0] (184-209) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-002-47 100 µl $450.00

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Protocol for Immunohistochemistry:
Tissue Sample mouse liver tissue
Fixative 10% Formalin
Embedding Paraffin
Negative control Pre-immuno serum
Pretreatment Intact
Blocking 2% Normal Goat Serum
Primary Antibody Rabbit Anti-FGF-21 (183-208) (Human) Serum (Catalog No.:H-002-47)
Optimal Dilution 1:250~500 (over night at 4oC)
Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification  ABC (Vector) (1:400, 30 min)
Detection system HRP
Substrate DAB (Sigma), 3 min
Counterstained Hematoxylin, 30 Sec
Catalog #H-002-47
Standard Size100 µl
Sequence(Cys) - Gln - Pro - Pro - Asp - Val - Gly - Ser - Ser - Asp - Pro - Leu - Ser - Met - Val - Gly - Pro - Ser - Gln - Gly - Arg - Ser - Pro - Ser - Tyr - Ala - Ser
SpeciesHuman
HostRabbit
ReconstitutionFor consistent and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum immediately before use.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Content This vial contains 100µl of rabbit anti-FGF-21 (183-208) (Human) serum in the lyophilized form.  
Recommended Dilution FactorDirect ELISA: 1:6400
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