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FGF-21 (26-47) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-002-46 100 µl $450.00

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Procotol for Immunohistochemistry 
 Tissue SampleHuman/Mouse liver tissue & Human hepatocellular carcinoma tissue
 Fixative10% Formalin
 EmbeddingParaffin
 Negative controlNo primary antibody
 PretreatmentN/A
 Blocking3% H2O2, 2% Normal Goat Serum
 Primary AntibodyRabbit Anti-FGF-21 (26-47) (Human) Serum (Catalog No.:H-002-46) 
 Optimal Dilution1:250~500 (over night at 4 OC)
 Secondary Antibody

Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min

 Amplification

Streptavidin-HRP (Vector), 1:400, 30 min

 Detection systemHRP
 SubstrateDAB (Sigma), 3 min
 CounterstainedHematoxylin, 30 Sec

Western blot analysis
 SampleRat liver tissue
 Electrophoresis15% SDS-PAGE gel, reducing condition
 Denatured sample 100'C for 5 min
 Positive controlrecombinant human FGF-21
 Blocking1% BSA in TBST
 Primary AntibodyRabbit Anti-FGF-21 (26-47) (Human) Serum (Catalog No.:H-002-46)
 Optimal Dilution1:500 (overnight at cold room)
 Secondary AntibodyRabbit anti-Mouse IgG, HRP conjugated
 SubstrateECL
 Positive Band~ 23 KDa

Catalog #H-002-46
Standard Size100 µl
SequenceCys - Gln - Ala - His - Pro - Ile - Pro - Asp - Ser - Ser - Pro - Leu - Leu - Gln - Phe - Gly - Gly - Gln - Val - Arg - Gln - Arg
SpeciesHuman
HostRabbit
ReconstitutionFor consistent and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum immediately before use.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 100µl of rabbit anti-FGF-21 (26-47) (Human) serum in the lyophilized form.
Recommended Dilution Factor

Direct ELISA: 1:6400
Immunohistochemistry (ABC):1:250-500
Western blot:1:350-500

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