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Angiotensin I (1-7) / Angiotensin II (1-7) (Human, Rat, Mouse, Canine) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-002-24 50 µl $450.00

Image

     

Protocol for Immunohistochemistry:

 Tissue Sample

 Rat & Mouse Heart

 Fixative

 10% formalin

 Embedding

 Paraffin

 Negative Control

 No primary antibody

 Pretreatment

 Intact

 Blocking

 2% Normal Goat Serum

 Primary Antibody

 Rabbit Angiotensin II (1-7) (Human) Antiserum (Catalog No.:H-002-24)

 Optimal Dilution

 1:100

 Secondary Antibody

 Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)

 Amplification

 ABC (Vector) (1:400, 30 min)

 Detection System

 HRP

 Substrate

 DAB (Sigma), 3 min

 Counterstained

 Hematoxylin, 30 Sec

Catalog #H-002-24
Standard Size50 µl
SequenceAsp - Arg - Val - Tyr - Ile - His - Pro
SpeciesHuman
HostRabbit
ReconstitutionFor consistent and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 50µl of rabbit anti- Angiotensin II (1-7) (Human) serum in the lyophilized form.
Recommended Dilution Factor

Immunofluorescence:  1:200 
PAP or ABC:  1:500

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