中文网站正在持续更新中,请密切关注我们康肽生物的最新动态,或点击访问右上角的英文官方网站 www.phoenixpeptide.com
PHOENIX PHARMACEUTICALS, INC. TOP HOME PAGE
Top » Catalog » Antibodies » For Immunohistochemistry » H-002-12 English Version | My Account | 联系我们 | China



 多肽



 标记多肽 



 多肽激素文库



 抗体 
   免疫组化抗体
   纯化免疫球蛋白 
   抗体标记 



 免疫试剂盒 



 生物标志物阵列 



 多肽样品检测



 自定义肽链合成及GMP



 产品目录索取



 样品准备



 提问和解答


Angiotensin II (Human, Rat, Mouse, Canine) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-002-12 50 µl $175.00

Image

   

Protocol for Immunohistochemistry:

 Tissue Sample

 Rat Kidney

 Fixative

 10% formalin

 Embedding

 Paraffin

 Negative Control

 No primary antibody

 Pretreatment

 Pronase

 Blocking

 3% H2O2, 2% Normal Goat Serum

 Primary Antibody

 Rabbit Anti-Angiotensin II Antiserum (Catalog No.:H-002-12)

 Optimal Dilution

 1:2000 (1hour at RT)

 Secondary Antibody

 Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)

 Amplification

 ABC (Vector) (1:400, 30 min)

 Detection System

 HRP

 Substrate

 DAB (Sigma), 3 min

 Counterstained

 Hematoxylin, 30 Sec

Catalog #H-002-12
Standard Size50 µl
SequenceAsp - Arg - Val - Tyr - Ile - His - Pro - Phe
SpeciesHuman, Rat, Mouse, Porcine, Canine
Specific SpecieGuinea Pig, Rabbit
HostRabbit
ReconstitutionFor consistent and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum immediately before use. Reconstituted antibody can also be aliquotted (5~10µl) and stored frozen at
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 50µl of rabbit anti- Angiotensin II (Human) serum in the lyophilized form.
Recommended Dilution FactorABC:                             1:100, 1:200
Western Blot:               1:200
Immunocytochemistry- 1:200 (Invest Ophthalmol Vis Sci. 2007 Jul;48(7):3301-11.)
Cross Reactivity
 Peptide %Cross-reactivity 
 Angiotensin II (Human)  100
 Angiotensin III (Human)  100
 Angiotensin II (1-4) (Human)  0.1
 Angiotensin II (5-8) (Human)  0.001
 Pre-Angiotensin (1-14) 1
 Angiotensin I (Human) 1.7
 ACTH (Human) 0
 Endothelin-1 (Human, Porcine, Rat) 0
 [Arg8]-Vasopressin  0
Alphabet_A_
Please click here or click the reference tab on the top to see more articles that use this product.
分类搜索
关键字搜索
按字母搜索
A B C D E F G H I J K L M N
O P Q R S T U V W X Y Z

Copyright © 2024 PHOENIX BIOTECH