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Adiponectin Precursor [Cys0] (222-244) C-terminal (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-028-51 50 µl $450.00

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Protocol for Immunohistochemistry: 
 Tissue Sample Rat liver & visceral white fat tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment Intact
 Blocking 2% Normal Goat Serum
 Primary Antibody Anti-Adiponectin, (222-244), Cys0 (Human) serum (H-028-51)
 Optimal Dilution 1: 50-100 (for liver), 1:500~1000 (overnight hour at 4 0C for fat)
 Secondary AntibodyGoat anti-Rabbit IgG, HRP conjugated
 Amplification  ABC (Vector)
 Detection system HRP
 Substrate DAB (Sigma)
 Counterstained Hematoxylin

Catalog #H-028-51
Standard Size50 µl
SequenceAsn - Gly - Leu - Tyr - Ala - Asp - Asn - Asp - Asn - Asp - Ser - Thr - Phe - Thr - Gly - Phe - Leu - Leu - Tyr - His - Asp - Thr - Asn
SpeciesHuman
HostRabbit
ReconstitutionFor best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 50µl of Rabbit Anti-[Cys0]-Adiponectin (222-244) (Human) Serum in the lyophilized form.
Recommended Dilution FactorWestern Blotting: 1: 100-200
IHC: 1: 50-100
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