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Farnesoid X Receptor (FXR) (446-476) (Human, Rat, Mouse) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-001-65 50 µl $450.00

Image

     

Protocol for Immunohistochemistry

Tissue Sample

Rat Liver Tissue

Fixative

10% Formalin

Embedding

Paraffin

Negative control

No primary antibody

Pretreatment

Intact

Blocking

2% Normal Goat Serum

Primary Antibody

Rabbit Anti-FXR (446-476) (Human, Mouse, Rat) Serum (Catalog No.:H-001-65)

Optimal Dilution

1:200 (1hour at RT)

Secondary Antibody

Goat Anti-Rabbit IgG, Biotinylated (1:400, 30 min)

Amplification 

ABC (Vector) (1:400, 30 min)

Detection system

HRP

Substrate

DAB (Sigma), 3 min

Counterstained

Hematoxylin, 30 Sec


Catalog #H-001-65
Standard Size50 µl
SequenceThr - Phe - Asn - His - His - His - Ala - Glu - Met - Leu - Met - Ser - Trp - Arg - Val - Asn - Asp - His - Lys - Phe - Thr - Pro - Leu - Leu - Cys - Glu - Ile - Trp - Asp - Val - Gln
SpeciesHuman, Rat, Mouse
HostRabbit
ReconstitutionFor consistent and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 50µl of rabbit anti- Farnesoid X Receptor (FXR) (446-476) (Human, Rat, Mouse) serum in the lyophilized form.
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