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GPR-54 (375-396) (Mouse) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-001-64 100 µl $450.00

Image

Protocol for Immunohistochemistry:

Tissue Sample

Rat hypothalamus tissue

Fixative

10% Formalin

Embedding

Paraffin

Negative control

No primary antibody

Pretreatment

Intact

Blocking

2% Normal Goat Serum

Primary Antibody

Rabbit Anti-GRP54 (375-396) (Mouse) Serum (Catalog No.:H-001-64)

Optimal Dilution

1:50-100 (1hour at RT)

Secondary Antibody

Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)

Amplification 

ABC (Vector) (1:400, 30 min)

Detection system

HRP

Substrate

DAB (Sigma), 3 min


Catalog #H-001-64
Standard Size100 µl
SequenceThr - Pro - Glu - Pro - Gly - Asn - Pro - Val - Val - His - Ser - Pro - Ser - Val - Gln - Asp - Glu - His - Thr - Ala - Pro - Leu
SpeciesMouse
HostRabbit
ReconstitutionFor consistent and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 100µl of rabbit anti-GPR54 (375-396) (Mouse) serum in the lyophilized form. The HPLC purified standard peptide, GPR54 (375-396) (Mouse) can be detected with this antiserum (1: 6400, 0.156ug/ml) in ELISA system.
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