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Intelectin / Omentin (163-199) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-028-85 100 µl $450.00

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Protocol for Immunohistochemistry: 
 Tissue Sample Rat, Mouse heart & Human visceral white fat tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment Intact
 Blocking 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-Intelectin /Omentin (163-199) (Human) Antiserum (Catalog No.:H-028-85)
 Optimal Dilution 1:500 (2hour at RT) conjugated
 Secondary Antibody Goat Anti-Rabbit IgG -HRP conjugated
 Substrate DAB(Sigma), 10"
 Counterstained Hematoxylin, 30 Sec

Catalog #H-028-85
Standard Size100 µl
SequenceAsn - Ser - Ser - Leu - Leu - Arg - Tyr - Arg - Thr - Asp - Thr - Gly - Phe - Leu - Gln - Thr - Leu - Gly - His - Asn - Leu - Phe - Gly - Ile - Tyr - Gln - Lys - Tyr - Pro - Val - Lys - Tyr - Gly - Glu - Gly - Lys - Cys
SpeciesHuman
HostRabbit
ReconstitutionFor best and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 100µl of Rabbit Anti- Intelectin / Omentin (163-199) (Human) Serum in the lyophilized form.
Recommended Dilution FactorImmunohistochemistry: 1: 500
Direct ELISA: 1: 3200
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