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Intelectin / Omentin (94-145) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-028-87 100 µl $450.00

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Protocol for Immunohistochemistry: 
 Tissue Sample Rat, Mouse heart & Human visceral white fat tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment intact
 Blocking 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-Intelectin /Omentin (94-145) (Human) Antiserum (Catalog No.:H-028-87)
 Optimal Dilution1:500 (overnight at 4 o C)
 Secondary Antibody Goat anti-Rabbit IgG, HRP conjugated
 Substrate DAB (Sigma), 10"
 Counterstained Hematoxylin, 30 Sec

Catalog #H-028-87
Standard Size100 µl
SequenceCys - Thr - Val - Gly - Asp - Arg - Trp - Ser - Ser - Gln - Gln - Gly - Ser - Lys - Ala - Asp - Tyr - Pro - Glu - Gly - Asp - Gly - Asn - Trp - Ala - Asn - Tyr - Asn - Thr - Phe - Gly - Ser - Ala - Glu - Ala - Ala - Thr - Ser - Asp - Asp - Tyr - Lys - Asn - Pro - Gly - Tyr - Tyr - Asp - Ile - Gln - Ala - Lys
SpeciesHuman
HostRabbit
ReconstitutionFor best and reproducible results, reconstitute with 100µl of distilled water, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 100µl of Rabbit Anti- Intelectin / Omentin (94-145) (Human) Serum in the lyophilized form.
Recommended Dilution FactorImmunohistochemistry: 1: 500
Direct ELISA: 1: 3200
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