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Adipo R1 (357-375) / Adiponectin Receptor Type 1 (357-375) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-001-44 50 µl $450.00

Image

 

Protocol for Immunohistochemistry
 Tissue Sample Rat Heart tissue 
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment Target retrieval 25 min (Steam)
 Blocking 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-Adiponectin Receptor 1 (357-375) (Human) Serum  (Catalog No.:H-001-44)
 Optimal Dilution 1:500 (1hour at RT)
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification  ABC (Vector) (1:400, 30 min)
 Detection system HRP
 Substrate DAB (Sigma), 3 min
 Counterstained Hematoxylin, 30 Sec
Catalog #H-001-44
Standard Size50 µl
SequenceAsn - Leu - Gln - Glu - Phe - Arg - Tyr - Gly - Leu - Glu - Gly - Gly - Cys - Thr - Asp - Asp - Thr - Leu - Leu
SpeciesHuman
HostRabbit
ReconstitutionFor consistent and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Content

This vial contains 50µl of rabbit anti- Adiponectin Receptor 1 (357-375) (Human) serum in the lyophilized form.

Recommended Dilution FactorImmunofluorescence: 1:200
PAP or ABC: 1:500
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