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INSL 3 C-Peptide (Mouse) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-035-47 50 µl $450.00

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Protocol for Immunohistochemistry: 
 Tissue Sample Rat testis
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment Target retrieval 25 min (Steam)
 Blocking 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-Insulin-like 3 C Peptide / Insulin-like 3 Prepro (58-105) (Mouse) Serum (Catalog No: H-035-47)
 Optimal Dilution 1:50 (1hour at RT)
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification  ABC (Vector) (1:400, 30 min)
 Detection system HRP
 Substrate DAB (Sigma), 3 min
 Counterstained Hematoxylin, 30 Sec

Catalog #H-035-47
Standard Size50 µl
SequenceThr - Gln - Pro - Val - Glu - Thr - Arg - Asp - Arg - Glu - Leu - Leu - Gln - Trp - Leu - Glu - Gln - Arg - His - Leu - Leu - His - Ala - Leu - Val - Ala - Asp - Val - Asp - Pro - Ala - Leu - Asp - Pro - Gln - Leu - Pro - Arg - Gln - Ala - Ser - Gln - Arg - Gln
SpeciesMouse
HostRabbit
ReconstitutionFor best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Reconstituted antibody can also be aliquotted (5~10µl) and stored frozen at
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