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H-035-62
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Relaxin 2 (Human) - Antibody for Immunohistochemistry
Catalog#
Standard size
Price
Order
H-035-62
100 µl
$450.00
Relaxin & Relaxin-Related Peptides
Relaxin 2 (Human)
Relaxin Evolution
GSS: Relaxin Like Peptide
Mini-Relaxin 2
Bioactive Human Relaxin-2
Antibody Production & Evaluation
Image
Protocol for Immunohistochemistry:
Tissue Sample
Human placenta, Rat & Mouse brain tissue
Fixative
10% Formalin
Embedding
Paraffin
Negative control
No primary antibody
Pretreatment
No
Blocking
2% Normal Goat Serum
Primary Antibody
Rabbit Anti- Relaxin 2 (Human) Antiserum (Catalog No.:H-035-62)
Optimal Dilution
1:200 (1hour at RT)
Secondary Antibody
Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
Amplification
ABC (Vector) (1:400, 30 min)
Detection system
HRP
Substrate
DAB (Sigma), 3 min
Counterstained
Hematoxylin, 30 Sec
Catalog #
H-035-62
Standard Size
100 µl
Species
Human
Host
Rabbit
Reconstitution
For best and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Reconstituted antibody can also be aliquotted (5~10µl) and stored frozen at
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