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Relaxin 2 (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-035-62 100 µl $450.00

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Protocol for Immunohistochemistry: 
 Tissue Sample Human placenta, Rat & Mouse brain tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment No
 Blocking 2% Normal Goat Serum
 Primary Antibody Rabbit Anti- Relaxin 2 (Human) Antiserum (Catalog No.:H-035-62)
 Optimal Dilution 1:200 (1hour at RT)
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification  ABC (Vector) (1:400, 30 min)
 Detection system HRP
 Substrate DAB (Sigma), 3 min
 Counterstained Hematoxylin, 30 Sec

Catalog #H-035-62
Standard Size100 µl
SpeciesHuman
HostRabbit
ReconstitutionFor best and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Reconstituted antibody can also be aliquotted (5~10µl) and stored frozen at
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