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Neuropeptide S, Prepro (23-67) (Mouse) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-005-99 100 µl $350.00

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Protocol for Immunohistochemistry
 Tissue Sample Rat and Mouse Brain Tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 PretreatmentTarget Retrieval 25 min (Steam)
 Blocking2% Normal Goat Serum, 3%H2O2
 Primary AntibodyRabbit anti-Neuropeptide S, Prepro (23-67) Serum (Catalog No.: H-005-99)
 Optimal Dilution1:500 (1 hour at RT )
 Secondary Antibody Goat Anti-Rabbit IgG, Biotinylated (1:400) 
 AmplificationABC (Vector)
 Detection systemHRP 
 SubstrateDAB (Sigma), 3 min
 CounterstainedHematoxylin
Catalog #H-005-99
FamilyNeuropeptide S
Standard Size100 µl
SequenceCys - Tyr - Pro - Val - Leu - Ser - Ser - Lys - Val - Pro - Gly - Lys - Pro - Asp - Tyr - Phe - Leu - Ile - Leu - Leu - Ser - Ser - Cys - Pro - Ala - Arg - Leu - Glu - Gly - Ser - Asp - Arg - Leu - Ala - Phe - Leu - Lys - Pro - Ile - Leu - Glu - Lys - Thr - Ser - Met Disulfide bridge Cys23 - Cys45
SpeciesMouse
HostRabbit
ImmunogenNeuropeptide S, Prepro (23-67) (Mouse)
Reconstitution100 ul
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Recommended Dilution FactorImmunohistochemistry (Rat Brain)     - 1:200
Immunohistochemistry (Mouse Brain) - 1:500
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%Neuropeptide S%

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