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INSL 7, C Peptide (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-035-52 100 µl $450.00

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Protocol for Immunohistochemistry: 
 Tissue Sample Rat, Mouse brain and testis tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment Intact
 Blocking 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-INSL7, C Peptide (Human) Serum (Catalog No.:H-035-52)
 Optimal Dilution 1: 500 (Overnight at 4oC)
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification  ABC (Vector) (1:400, 30 min)
 Detection system HRP
 Substrate DAB (Sigma), 3 min
 Counterstained Hematoxylin, 30 Sec
Catalog #H-035-52
Standard Size100 µl
SequenceSer - Asp - Ile - Leu - Ala - His - Glu - Ala - Met - Gly - Asp - Thr - Phe - Pro - Asp - Ala - Asp - Ala - Asp - Glu - Asp - Ser - Leu - Ala - Gly - Glu - Leu - Asp - Glu - Ala - Met - Gly - Ser - Ser - Glu - Trp - Leu - Ala - Leu - Thr - Lys - Ser - Pro - Gln - Ala - Phe - Tyr - Arg - Gly - Arg - Pro - Ser - Trp - Gln - Gly - Thr - Pro - Gly - Val - Leu - Arg - Gly - Ser - Arg
SpeciesHuman
HostRabbit
ReconstitutionFor consistent and reproducible results, reconstitute with 50ul of distilled water for the equivalent of undiluted antiserum, immediately before use. Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 100ul of rabbit anti- INSL7, C Peptide (H) serum in the lyophilized form.
Recommended Dilution FactorImmunohistochemistry (1:500) [Formalin/PFA-fixed paraffin-embedded sections]
Indirect ELISA (1: 6400)
Optimal dilutions/concentrations should be determined by the end user.
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