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EC SOD (Extracellular Superoxide Dismutase) [Cys0] (33-54) (Mouse) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-075-16 100 µl $450.00

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Protocol for Immunohistochemistry
 Tissue Sample rat kidney and heart tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody (pre-immuno serum)
 Pretreatment Intact
 Blocking 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-EC SOD (33-54) (Mouse) Serum (Catalog No.:H-075-16)
 Optimal Dilution 1:100~200 (1hour at RT)
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification  ABC (Vector) (1:400, 30 min)
 Detection system HRP
 Substrate DAB (Sigma), 3 min
 Counterstained Hematoxylin, 30 Sec
Catalog #H-075-16
Standard Size100 µl
SequenceCys - Leu - Asp - Pro - Val - Glu - Lys - Ile - Asp - Arg - Leu - Asp - Leu - Val - Glu - Lys - Ile - Gly - Asp - Thr - His - Ala - Lys
SpeciesMouse
HostRabbit
ImmunogenEC SOD (33-54) (Mouse)
ReconstitutionFor consistent and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 100µl of Rabbit Anti- [Cys0]-Extracellular Superoxide Dismutase (EC SOD) (33-54) (Mouse) Serum in the lyophilized form.
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