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MCH-2R [Cys0] (237-251) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-071-20 50 µl $450.00

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Protocol for Immunohistochemistry: 
 Tissue Sample Human ventral lateral hypothalamus
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment Target retrieval 25 min (Steam)
 Blocking 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-MCH-R2 (237-251), Cys0, (Human) Serum (Catalog No.:H-071-20)
 Optimal Dilution 1:2000 (1hour at RT)
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification  ABC (Vector) (1:400, 30 min)
 Detection system HRP
 Substrate DAB (Sigma), 3 min
 Counterstained Hematoxylin, 30 Sec

Catalog #H-071-20
Standard Size50 µl
SequenceCys - Asn - Pro - Ser - Val - Pro - Lys - Gln - Arg - Val - Met - Lys - Leu - Thr - Lys
SpeciesHuman
HostRabbit
ImmunogenMelanin-Concentrating Hormone Receptor 2 (MCH-2R) (237-251) (Human)
ReconstitutionFor consistent and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 50µl of Rabbit Anti-MCH-2R (237-251) (human) Serum in the lyophilized form.
Recommended Dilution FactorImmunofluorescence: 1:200
PAP or ABC: 1:500
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