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CCK (26-33) (Non-Sulfated) (Human, Rat, Mouse, Canine, Bovine) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-069-04 50 µl $175.00

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Protocol for Immunohistochemistry: 
 Tissue Sample Rat intestines / Human colon & colon cancer tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment N/A
 Blocking 3% H2O2, 2% Normal Goat Serum
 Primary Antibody

 Rabbit anti-CCK (26-33) (H,R,M) antibody (Cat. No.: H-069-04)

 Optimal Dilution 1:500 
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification

 Streptavidin-HRP (Vector), 1:400, 30 min

 Detection system HRP
 Substrate DAB (Sigma), 3 min
 Counterstained Hematoxylin, 30 Sec
Catalog #H-069-04
Standard Size50 µl
SequenceAsp - Tyr - Met - Gly - Trp - Met - Asp - Phe - NH2
SpeciesHuman, Rat, Mouse, Canine
HostRabbit
ReconstitutionFor best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 50µl of Rabbit Anti- Cholecystokinin Octapeptide (CCK 26-33) Serum in the lyophilized form.
Recommended Dilution FactorImmunofluorescence: 1:200
PAP or ABC: 1:500
Cross Reactivity
Peptide % Cross-reactivity 
CCK 26-33 (Non-Sulfated) 100
CCK-33 (Porcine) 100 
Gastrin-1 (Human) 100 
Big Gastrin-1 (Human)100 
CCK 26-33 (Sulfated) 78
CCK 27-33 63
CCK 30-33 14 
Pancreatic Polypeptide (Human) 
VIP (Human, Porcine, Rat) 
Alphabet_C_
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