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Hypothetical Protein XP_294524 (154-196) [pGlu154] Amide / QRFP-43 (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-048-78 50 µl $450.00

Image

Western Blot Analysis of Prepro-QRFP in rat brain tissue 
 Tissue Sample Rat brain tissue homogenate
 Electrophoresis 10% SDS-PAGE gel
 Negative control No primary antibody
 Blocking 1% BSA in TBST or 5% dry milk in TBST
 Primary Antibody Rabbit Anti-QRFP-43 (Human) Antiserum (Catalog No.:H-048-78)
 Optimal Dilution 1:500-~1000 (4 hour at RT, or overnight at 4' C)
 Secondary Antibody Donkey anti-Rabbit IgG whole (H+L), HRP conjugate(1:5000, 30 min)
 Detection system HRP
 Substrate SuperSignal Chemiluminescent Substrate

     

Protocol for Immunohistochemistry:
 Tissue Sample Rat or Mouse brain Tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment Intact
 Blocking 2% Normal Goat Serum
 Primary Antibody Rabbit Anti-QRFP-43 (Human) Antiserum (Catalog No.:H-048-78)
 Optimal Dilution 1:100 -200 (1 hour at RT)
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification
 ABC (Vector) (1:400, 30 min)
 Detection system HRP
 Substrate DAB (sigma), 30min
 Counterstained Hematoxylin, 30Sec

Catalog #H-048-78
Standard Size50 µl
SequencepGlu - Asp - Glu - Gly - Ser - Glu - Ala - Thr - Gly - Phe - Leu - Pro - Ala - Ala - Gly - Glu - Lys - Thr - Ser - Gly - Pro - Leu - Gly - Asn - Leu - Ala - Glu - Glu - Leu - Asn - Gly - Tyr - Ser - Arg - Lys - Lys - Gly - Gly - Phe - Ser - Phe - Arg - Phe - NH2
SpeciesHuman
Specific SpecieThis antibody is crossreactivity with human , rat and mouse QRFP-43 peptide at 100%. It has been tested in rat or mouse brain tissue.
HostRabbit
ReconstitutionFor best and reproducible results, reconstitute with 50µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
ContentThis vial contains 50µl of Rabbit Anti- QRFP-43-Amide (Human) Serum in the lyophilized form.
Recommended Dilution Factor1: 500 ~ 1000 (western blot)
1: 6400 (indirect ELISA)
1: 100 ~ 200 (Immunohistochemistry-ABC)
Alphabet_H_
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