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Salusin-alpha (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-010-67 100 µl $450.00

Image

    

    

Protocol for Immunohistochemistry
 Tissue SampleHuman stomach cancer tissue or adrenal medullary tissue
 Fixative10% Formalin 
 EmbeddingParaffin 
 Negative controlNo primary antibody 
 PretreatmentN/A
 Blocking

3% H2O2, 2% Normal Goat Serum

 Primary Antibody

Rabbit Anti-Salusin-alpha (Human) AntiSerum (Catalog No.: H-010-70)

 Optimal Dilution

1:500, 1 hour at RT

 Secondary AntibodyGoat anti-Rabbit IgG, Biotinylated (1:400, 30 min) 
 Amplification

Streptavidin-HRP (Vector), 1:400, 30 min

 Detection systemHRP 
 SubstrateDAB (Sigma), 3 min 
 CounterstainedHematoxylin, 30 Sec
Catalog #H-010-67
FamilySalusin
Standard Size100 µl
SequenceCys0 - Ser - Gly - Ala - Leu - Pro - Pro - Ala - Pro - Ala - Ala - Pro - Arg - Pro - Ala - Leu - Arg - Ala - Gln - Arg - Ala - Gly - Pro - Ala - Gly - Pro - Gly - Ala - Lys - NH2
SpeciesHuman
HostRabbit
ImmunogenSalusin-alpha (Human)
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Alphabet_S_

%010-70%;%010-67%;%010-73%

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