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Gastrin Releasing Peptide (GRP), Pro (80-97) [Tyr0] (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-027-36 100 µl $275.00

Image    
Protocol for Immunohistochemistry
 Tissue Sample

Human Lung Cancer Tissue

 Fixative10% Formalin 
 EmbeddingParaffin 
 Negative controlNo primary antibody 
 PretreatmentN/A
 Blocking3% H2O2, 2% Normal Goat Serum
 Primary AntibodyRabbit Anti-GRP , Pro(80-97) (Human) (Catalog No.: H-027-36)
 Optimal Dilution

1:500

 Secondary AntibodyGoat anti-Rabbit IgG, Biotinylated (1:400, 30 min) 
 Amplification

Streptavidin-HRP (Vector), 1:400, 30 min

 Detection systemHRP 
 SubstrateDAB (Sigma), 3 min 
 CounterstainedHematoxylin, 30 Sec
Catalog #H-027-36
FamilyGRP
Standard Size100 µl
Sequence(Tyr) - Ala - Leu - Gly - Asn - Gln - Gln - Pro - Ser - Trp - Asp - Ser - Glu - Asp - Ser - Ser - Asn - Phe - Lys
SpeciesHuman
HostRabbit
ImmunogenGastrin Releasing Peptide (GRP), Pro (80-97) (Human)
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Alphabet_G_

%027-36%;%027-35%

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