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Synaptotagmin IV (73-97) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-008-64 100 µl $275.00

Image 
Protocol for Immunohistochemistry
 Tissue Sample

Human brain tissues

 Fixative10% Formalin 
 EmbeddingParaffin 
 Negative controlNo primary antibody 
 PretreatmentN/A
 Blocking3% H2O2, 2% Normal Goat Serum
 Primary AntibodyRabbit Anti-Synaptotagmin IV (73-97) (Human) Antibody (Catalog No.: H-008-64)
 Optimal Dilution

1:500, 1 hour at RT

 Secondary AntibodyGoat anti-Rabbit IgG, Biotinylated (1:400, 30 min) 
 Amplification

Streptavidin-HRP (Vector), 1:400, 30 min

 Detection systemHRP 
 SubstrateDAB (Sigma), 3 min 
 CounterstainedHematoxylin, 30 Sec
Catalog #H-008-64
FamilySynaptotagmin IV
Standard Size100 µl
SequencePhe - Gly - Ala - Asp - Asp - Lys - Asn - Glu - Val - Lys - Asn - Lys - Pro - Ala - Val - Pro - Lys - Asn - Ser - Leu - His - Leu - Asp - Leu - Glu
SpeciesHuman
HostRabbit
ImmunogenSynaptotagmin IV (73-97) (Human)
ReconstitutionFor best and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Recommended Dilution FactorImmunohistochemistry - 1:500
Alphabet_S_

%008-64%;%008-65%

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