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Augurin, Prepro (108-132) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-012-23 100 µl $275.00

Image    
Protocol for Immunohistochemistry: 
 Tissue Sample

 Mouse Brain tissues

 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment N/A
 Blocking 3% H2O2, 2% Normal Goat Serum
 Primary Antibody

 Rabbit Anti-Augurin, Prepro (108-132) (H) Antiserum (Catalog No.: H-012-23)

 Optimal Dilution 1:500 , 1 hour at RT
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification

 Streptavidin-HRP (Vector), 1:400, 30 min

 Detection system HRP
 Substrate DAB (Sigma), 3 min
 Counterstained Hematoxylin, 30 Sec
Catalog #H-012-23
FamilyAugurin
Standard Size100 µl
SequenceAsp - Arg - Asn - Gly - His - Glu - Tyr - Tyr - Gly - Asp - Tyr - Tyr - Gln - Arg - His - Tyr - Asp - Glu - Ser - Ala - Ile - Gly - Pro - Arg
SpeciesHuman
HostRabbit
ImmunogenAugurin, Prepro (108-132) (Human)
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Alphabet_A_

%012-23%;%012-22%;%012-21%;%012-24%

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