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PACAP (6-27) (Human, Rat, Ovine) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-052-04 100 µl $350.00

Image

    

Protocol for Immunohistochemistry: 
 Tissue Sample Human Brain Tissue
 Fixative 10% Formalin
 Embedding Paraffin
 Negative control No primary antibody
 Pretreatment N/A
 Blocking 3% H2O2, 2% Normal Goat Serum
 Primary Antibody

 Rabbit anti-PACAP (6-27) (H,R,O) Antibody (Cat. No.: H-052-04)

 Optimal Dilution 1:500 
 Secondary Antibody Goat anti-Rabbit IgG, Biotinylated (1:400, 30 min)
 Amplification

 Streptavidin-HRP (Vector), 1:400, 30 min

 Detection system HRP
 Substrate DAB (Sigma), 3 min
 Counterstained Hematoxylin, 30 Sec
Catalog #H-052-04
Standard Size100 µl
SequencePhe - Thr - Asp - Ser - Tyr - Ser - Arg - Tyr - Arg - Lys - Gln - Met - Ala - Val - Lys - Lys - Tyr - Leu - Ala - Ala - Val - Leu - NH2
SpeciesHuman, Rat, Ovine
HostRabbit
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
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