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CCK (46-69) (Human) - Antibody for Immunohistochemistry

Catalog# Standard size Price Order
H-069-27 100 ul $350.00

Image
Procotol for Immunohistochemistry 
 Tissue SampleHuman colon cancer tissues
 Fixative10% Formalin
 EmbeddingParaffin
 Negative controlNo primary antibody
 PretreatmentN/A
 Blocking3% H2O2, 2% Normal Goat Serum
 Primary AntibodyRabbit anti-CCK (46-69) (Human) antibody (Cat. No.: H-069-27)
 Optimal Dilution1:500
 Secondary Antibody

Goat Anti-Rabbit IgG, Biotinylated (1:400), 30 min

 Amplification

Streptavidin-HRP (Vector), 1:400, 30 min

 Detection systemHRP
 SubstrateDAB (Sigma), 3 min
 CounterstainedHematoxylin, 30 Sec
Catalog #H-069-27
FamilyCCK
Standard Size100 ul
SequenceVal - Ser - Gln - Arg - Thr - Asp - Gly - Glu - Ser - Arg - Ala - His - Leu - Gly - Ala - Leu - Leu - Ala - Arg - Tyr - Ile - Gln - Gln - Ala
SpeciesHuman
HostRabbit
ImmunogenCCK (46-69) (Human)
ReconstitutionFor best and reproducible results, reconstitute with 100µl of distilled water for the equivalent of undiluted antiserum, immediately before use. Do not refreeze any unused portions.
Storage ConditionPlease store the lyophilized antibody at -20°C upon receipt. For optimal results, use the antibody immediately after reconstitution. Once reconstituted, the antibody is stable for up to three (3) days at 4°C. For longer-term storage up to three (3) months, prepare small aliquots of the reconstituted antibody and freeze at -20°C or -80°C. Repeated freeze-thaw cycles should be strictly avoided.
Alphabet_C_

%069-27%

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