Lot No.: 426843
Product: Adiponectin Recombinant, Globular Form (Human)
Molecular Weight: 19 kDa
This vial contains 100 mg of protein.
Supplied: The sterile filtered solution was lyophilized from 20mM Tris, 20 mM NaCl, pH 8.0
Tag: 6 x His Tag on N-Terminal
Purity: >95% by SDS-Page analysis
Solubility and Reconstitution:
The lyophilized adiponectin (human) should be reconstituted in water to a concentration of 100 µg/ml. Allow to set at least 30 minutes at 4-8˚C, mix well.
Storage: The lyophilized powder is stable at room temperature for a few weeks but it is best stored desiccated at -20˚C to -70˚C. Reconstituted Adiponectin can be stored at 4˚C for one week or in working aliquots at -20˚C to
-70˚C for long term storage.
AVOID FREQUENT FREEZE THAW CYCLE.
ELISA, Western Blot Analysis, Cell Biology.
Adiponectin is very important adipokine involved in the control of fat metabolism and
Insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory
activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal
muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes α-TNF
by negatively regulating its expression in various tissues such as liver and
macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B
signaling through a c-AMP-dependent pathway. May play a role in cell growth,
angiogenesis and tissue remodeling by binding and sequestering various factors with
distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.