Description: TNF-alpha is a pleiotrophic pro-inflammatory cytokine secreted by various cells including adipocytes, activated monocytes, macrophages, B cells, T cells and fibroblasts. It belongs to the TNF family of ligands and signals through two receptors, TNFR1 and TNFR2. TNF-alpha is cytotoxic to a wide variety of tumor cells and is an essential factor in mediating the immune response against bacterial infections. TNF-alpha also play a role in the induction of septic shock, auto immune diseases, rheumatoid arthritis, inflammation and diabetes. Recombinant human TNF-alpha is a soluble 157 amino acid protein (17.4 kDa) which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

Supplied: The sterile filtered solution was lyophilized from 3 mM Tris, pH 8.0

Source: E.Coli

Reactivity: The ED50 as determined by the cytolysis of murine L929 cells in the presence of Acitomyocin D is ≤0.05 ng/ml, corresponding to a specific activity of 2 x 107 units/mg

Precautions: For Research Use Only. Not for use in diagnostic or therapeutic procedures.